Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Genome wide analysis and clinical correlation of chromosomal and transcriptional mutations in cancers of the biliary tract

doi: 10.1186/1756-9966-28-62

Figure Lengend Snippet: Real-Time PCR Based Validation of Gene Expression Findings . To confirm the gene expression changes in biliary tract cancers identified on microarray analysis, selected genes were tested in tumor and control specimens by RT PCR and normalized to HRPT which is similarly expressed in tumors and normal biliary epithelia. Results are shown for (g) IL6 , (h) FOSB , (i) CDKN1C , (j) NR4A2 , and (k) DLC .

Article Snippet: 1 ug of total RNA was reverse-transcribed using the Thermoscript RT-PCR system (Invitrogen) at 52°C for 1 h. 20 ng of resultant cDNA was used in a Q-PCR reaction using an iCycler (Biorad, Hercules, California, USA) and pre-designed TaqMan ABI Gene expression Assays (Hs00270424_m1 for CCNB2 , Hs00938777_m1 for CDC2 , Hs00175938_m1 for CDKN1C , Hs01665258_m1 for DLC1 , Hs01547109_m1 for FOSB , Hs99999032_m1 for IL6 , s01118813_m1 for NR4A2 , Hs00971643_g1 for SRD5A1 , Hs01014001_m1 for STAT1 , Hs00426591_m1 for TYMS , Hs00197374_m1 for UBD ).

Techniques: Real-time Polymerase Chain Reaction, Biomarker Discovery, Gene Expression, Microarray, Control, Reverse Transcription Polymerase Chain Reaction

Journal: Cells

Article Title: Ethanol Induces Neuroinflammation in a Chronic Plus Binge Mouse Model of Alcohol Use Disorder via TLR4 and MyD88-Dependent Signaling

doi: 10.3390/cells12162109

Figure Lengend Snippet: TaqMan gene expression assays. 20X primer/probe sets (FAM-MGB) were purchased from Thermo Fisher Scientific, Cat. #4331182, and were used at a final concentration of 1X for qRT-PCR. Assays were selected to span an exon–exon junction where possible.

Article Snippet: Fosb , Mm00500403_m1.

Techniques: Gene Expression, Concentration Assay

Journal: Cells

Article Title: Ethanol Induces Neuroinflammation in a Chronic Plus Binge Mouse Model of Alcohol Use Disorder via TLR4 and MyD88-Dependent Signaling

doi: 10.3390/cells12162109

Figure Lengend Snippet: Two-way ANOVA for FosB and JunB in the cerebellum of wild-type, TLR4-, MyD88-, and TRIF-deficient mice. GraphPad Prism 9 was utilized to perform ordinary two-way analysis of variance (ANOVA) as described in the Methods. The main effects of treatment (control or ethanol) and genotype (wild-type or TLR4_KO, MyD88_KO, or TRIF_KO) and their interaction were evaluated. p values < 0.05 were considered significant. *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant.

Article Snippet: Fosb , Mm00500403_m1.

Techniques: Control

Journal: Cells

Article Title: Ethanol Induces Neuroinflammation in a Chronic Plus Binge Mouse Model of Alcohol Use Disorder via TLR4 and MyD88-Dependent Signaling

doi: 10.3390/cells12162109

Figure Lengend Snippet: Effects of ethanol on FosB ( A ) and JunB ( B ) mRNA expression in the cerebellum of wild-type, TLR4-, MyD88-, and TRIF-deficient mice. Mice were given access to a control or ethanol liquid diet, as described in the Methods, and relative mRNA expression was measured using qRT-PCR. Results are expressed as ∆∆CT fold change relative to control for each genotype. Individual values for each sample were plotted and error bars denote mean +/− SEM. *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant.

Article Snippet: Fosb , Mm00500403_m1.

Techniques: Expressing, Control, Quantitative RT-PCR